High sensitivity ，Rapid Boldenone ELISA Kit

Extraction Diluent

Mix 50mL of methanol with 950mL of distilled water, ready for use.

Meat(Fish,Shrimp,Beef,Chicken,Pork)

• Homogenize a reasonable amount of sample with a suitable mixer.
• Weigh out 1g of the homogenize sample into a suitable vial and mix thoroughly with 4mL of Extraction Diluent, vigorously vortex for 2min.
• Centrifuge it at 4,000 x g for 5 minutes.
• Use 50μL of the supernatant per well for the assay.

Note: Dilution Factor :4

Milk

• High-fat milk should be defatted by centrifuging;
• Pipette 2mL of the milk sample and add 0.5mL of methanol, vortex for 15s
• Use 50μL per well for the assay.

Note:Dilution Factor:1.25

Urine

• Centrifuge 1 mL of the urine sample at 4,000 x g for 5 minutes.
• Pipette 0.5mL of the urine supernatant and mix with 0.4mL of distilled water and 0.1mL of methanol, vortex for 15s;
• Use 50μL per well for the assay.

Note: Dilution Factor:2

Plasma/Serum

• Pipette 2mL of the homogenize sample into a suitable vial and mix with 0.5mL of methanol ,vortex for 15s.
• Use 50μL per well for the assay.

Note: Dilution Factor: 1.25